Sorry for the delay, I was away last week.
Which IDE are you using? The Chrome App, or espruino.com/ide ?
Had you connected a different device to the IDE between times? If you don't connect outside of bootloader mode first then the IDE doesn't know what type of device it should be using.
It might be worth restarting your PC and trying again, just in case windows has got confused and thinks some other application is using the COM port.
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