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I'm afraid not - software SPI isn't fast enough (you'd have a break between individual bits as Espruino went away to figure out what the next bit of data was).
The hardware SPI only works because quite a bit of effort has been put in to try and ensure that is can be kept topped up with data.
Is there a way to get WS2812 running on software spi ?
send4bit, which is used in examples is not supported for software spi